2371. Trouble going from plates to liquid culture - (reply: 2)
2372. RNA quality from total blood - (reply: 6)
2373. RNA extraction from TissueTek embedded tissue - (reply: 1)
2374. condition for keeping integrity of RNA for RT-PCR - (reply: 1)
2375. homologous recombination - (reply: 1)
2376. U6, H1 siRNA - (reply: 1)
2377. Different sizes of PCR products from different competent cells - (reply: 4)
2378. Designing PCR Primers - (reply: 3)
2379. sequencing - which primer should I trust? (reply: 4)
2380. Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (reply: 4)
2381. Purification and cloing of large fragments - cloning (reply: 3)
2382. Need Help with a Calculation - (reply: 5)
2383. Extract DNA from dead bacteria - (reply: 4)
2384. Help- degenerate primer design - (reply: 4)
2385. Long PCR on a cosmid vector - Help needed to run PCR (reply: 3)
2386. amount of extrated RNA - how much do u get (reply: 2)
2387. A basic question about DNA extraction - (reply: 2)
2388. Question about phosphorylation and ligation - (reply: 1)
2389. Does set up PCR mix days prior the amplification affects the results? - (reply: 2)
2390. the results of allele specific PCR is the other way around - I dont know why (reply: 1)
2391. start codon variants - (reply: 1)
2392. Chemically Modified Taq - Taq Polymerase (reply: 3)
2393. No electropherogram peak - (reply: 1)
2394. Plasmid for qPCR standard curve - (reply: 3)
2395. Purification after oligos phosphorylation? - (reply: 1)
2396. racepcr - waht is it (reply: 4)
2397. protein-DNA interactions - (reply: 2)
2398. TOPO >10Kb into pENTR-D-TOPO? - (reply: 2)
2399. Klenow Fill-in - How to inactivate it (reply: 4)
2400. role of MgCl2 and DTT in PCR - (reply: 3)

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