3061. to extract DNA from agarose gel - (reply: 11)
3062. PCR using a long (100nucleotides) and a short (20 nucleotides) primer. Problem: - (reply: 7)
3063. Suggestions for oligo modifications - (reply: 6)
3064. PCR quickchange with high Tm oligos - help needed (reply: 2)
3065. PCR mixing - First tubes generally work, last ones generally don't. (reply: 3)
3066. Sequencing Analysis - (reply: 7)
3067. DNA isolation from feathers - (reply: 2)
3068. RNA extraction from cells grown in matrigel - (reply: 1)
3069. size of probes for northern blot and other questions - end labelling of 20 mer oligonucleotides (reply: 3)
3070. stable transfectant - is there a way to make stables without antibiotic resistance? (reply: 2)
3071. PCR question, need help please! - (reply: 5)
3072. help: why PGK chosen as inner control? - (reply: 2)
3073. What happens to my ligation system? - Very Very urgent! (reply: 3)
3074. How QBI SP163 translational enhancer works? - (reply: 3)
3075. Storage of PCR product? - (reply: 1)
3076. Breakpoints in chromosomal aberrations - Are there "preferred" sequences? (reply: 2)
3077. ApaI / Not I double digestion problem - (reply: 5)
3078. DH5alpha transformation: protocol - (reply: 5)
3079. Stripping DNA probe from dna dot blot - (reply: 1)
3080. how to find a binding site in genes - help, please (reply: 5)
3081. Is competent cell still good in -80oC for 16 months? - got a different plasmid after plasmid prep (reply: 3)
3082. specific loci not present depending on DNA extraction method? - DNA extraction and succesful PCR (reply: 1)
3083. DNA agarose gels - trouble shoot weird gels (reply: 7)
3084. Is DNAse treatment essential during RNA extraction - (reply: 1)
3085. Spurios errors while cloning - cloning mutations (reply: 4)
3086. Plate storage & picking colonies - How much time do I have? (reply: 4)
3087. Bioanalyzer: the new series II kit - Agilent 2100 Bioanalyzer (reply: 2)
3088. Vector question - (reply: 6)
3089. Exposing southern gel to UV before transfer - (reply: 1)
3090. transgenic mouse analysis - I have generated a transgenic mouse for a protein that is expressed in the brain (reply: 2)

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