541. when does pcr products degrade like this? - images attached (reply: 7)
542. Choosing Primer: random or Olig-dt ? - (reply: 2)
543. RPS18 = 18s rRNA ? - (reply: 2)
544. problem in PCR pdt size - (reply: 4)
545. Expression Vector sequencing problem - (reply: 1)
546. how to synthesize the pcr primers - (reply: 3)
547. designing degenerate primer - (reply: 2)
548. troubleshooting with site directed mutagenesis - (reply: 2)
549. Solubility of Oligonucleotides? - (reply: 2)
550. Washing the spin columns from PCR purekit - (reply: 1)
551. Kozak sequence - (reply: 1)
552. incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
553. Cloning with methylated vector - (reply: 2)
554. pPICZA recombination in Ecoli - (reply: 7)
555. how to design pcr primers - (reply: 7)
556. RNA extraction from skin tissue - (reply: 2)
557. RT-PCR primer design and in silico PCR - (reply: 4)
558. DNA molar calculation - (reply: 3)
559. isolation of genomic DNA - (reply: 1)
560. RNA pellet can't dissolve in ethanol - (reply: 3)
561. how to know RNA concentration? - (reply: 4)
562. PCR yield, can assess by absorbance without electroforesis? - (reply: 2)
563. A260/280 - (reply: 3)
564. Checking mRNA expression by RT-PCR - (reply: 2)
565. Help with DIG UTP Pobe Construction In Vitro Transcription - (reply: 1)
566. Problem in RT-PCR - getting a band in the negative control (reply: 4)
567. methylation affects ligation? - (reply: 2)
568. DNA extraction vs purification - help ! (reply: 2)
569. Northern - really stuck here! - (reply: 3)
570. primer design - over expression (reply: 1)

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