5101. Cloning problem - Digestion, ligation and transformation (reply: 6)
5102. influence of MgCl2 in PCR - (reply: 7)
5103. Colony PCR for clone screen - Colony PCR before sending sequence (reply: 4)
5104. cDNA quantification - (reply: 1)
5105. Alignment tool - (reply: 3)
5106. partial digestion - restriction conditions (reply: 2)
5107. polishing.... followed by ligation - I HAVE BEEN STRUCK FOR THE FIRST TIME ... (reply: 1)
5108. Vector Vs Host - PGL2 Vs DH5alpha (reply: 1)
5109. RT-PCR - (reply: 1)
5110. Sequencing problems!! - homopolymeric region and slippage (reply: 8)
5111. RNA Isolation - (reply: 3)
5112. Difference between Luria Agar and Luria-Bertani - Luria Agar and Luria-Bertani (reply: 2)
5113. ligation question - ligation (reply: 3)
5114. please tell me how to eliminate interfering band ? - (reply: 1)
5115. LoxP - (reply: 2)
5116. gel stain and gel extraction - crystal violet (reply: 1)
5117. Increase Tansformation Efficiency for DH5alpha - I got only 5.8x10-5. Can any1 give some tips? (reply: 1)
5118. Chloroform-isoamyl extraction problem - Chloroform-isoamyl extraction problem (reply: 4)
5119. How to prepare enough plasmid for transfection? - (reply: 3)
5120. dNTPs - PCR (reply: 1)
5121. ligation question - ligation of size-selected DNA (reply: 4)
5122. Problem in the Digestion - Bamh! and EcoR1 (reply: 3)
5123. DNA extraction from plasma/serum - (reply: 2)
5124. Vector- Insert Ratio in Ligation - (reply: 2)
5125. Why is DTT used in reverse transcription (RT) reaction? - (reply: 9)
5126. Tripple Ligation - (reply: 7)
5127. northern transfer - semi dry method (reply: 11)
5128. Cloning into GFP vector - (reply: 2)
5129. cloning of long viral fragment I - (reply: 2)
5130. Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)

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