2221. Contradicting Tm - (reply: 3)
2222. cycling parameters for PCR - (reply: 4)
2223. Can primers go bad and give a smear in PCR? - (reply: 12)
2224. primer Tm - (reply: 10)
2225. PCR product 1200bp - (reply: 3)
2226. Stable Clone establishment using Hygromycin or Puromycin vectors - (reply: 3)
2227. A 270 for RNA - (reply: 1)
2228. Homologous recombination - (reply: 1)
2229. PCR cloning strategy - (reply: 4)
2230. Site-Directed Mutagenesis (megaprimer) of internal sequence - (reply: 5)
2231. in vitro transcription - transcription of long transcripts (reply: 1)
2232. problems with yields from qiagen spin mini prep kit - (reply: 21)
2233. avicel medium for RNA extraction - crushing of avicel (reply: 1)
2234. smear in degenerate PCR - (reply: 3)
2235. dUTP s - Explanation ! (reply: 1)
2236. strange sequencing / cloning problem with AvrII-site - (reply: 2)
2237. What is the function of NADPH in the reaction system? - (reply: 2)
2238. Proteinase K - How to use it? (reply: 2)
2239. Single Codon Deletion - SOE PCR - (reply: 3)
2240. QuickChange primers self-made - (reply: 4)
2241. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
2242. running DNA on gel - (reply: 2)
2243. Tn10 based Transposone mutagenesis - pLBT::mini-Tn10:lac:kan (reply: 1)
2244. how to get specific amplfication of a gene that is extremely similar to another - (reply: 7)
2245. How to select candidate TFs for future study from the prediction results? - (reply: 4)
2246. cloning/restriction analyses MAJOR PROBLEM - (reply: 2)
2247. protein concentration - bradford method (reply: 9)
2248. PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
2249. K to R mutation for kinase activity change - (reply: 1)
2250. Genomic DNA cutting - (reply: 12)

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