871. Sproadic PCR failures - (reply: 5)
872. Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
873. Fate of plasmid - How to detect plasmid DNA on host chromosome (reply: 7)
874. how to check gDNA quality or amount - as title (reply: 3)
875. 3' RACE with just 10ng starting total RNA - (reply: 2)
876. Can anyone recommend a good thermocycler? - (reply: 3)
877. Miniprep DNA - (reply: 2)
878. Housekeeping genes for rat brain - (reply: 4)
879. Will GFP interferes with luciferase assay result? - (reply: 3)
880. some primer dilution calculations - (reply: 7)
881. How to search plasmid sequence for restriction sites and resistance genes? - (reply: 4)
882. Image contrast adjustments for quantitated Northern blot pictures - (reply: 4)
883. Band Intensity - Linear or Exponential? PCR Products - (reply: 4)
884. To whom working with DNA sequencing - (reply: 2)
885. linearized vector purification from TAE agarose gel - (reply: 5)
886. PCR - reduced binding efficiency with lower Tm? - (reply: 2)
887. CLONING LONG INSERT RATIO INS:VEC - (reply: 4)
888. Improving PCR specificity? - (reply: 2)
889. Purified PCR product - (reply: 2)
890. all the DNA gone after digestion - (reply: 8)
891. Difficulty resuspending DNA after precipitation - (reply: 7)
892. PCR product too short - (reply: 6)
893. help with His-tagged vector construct - (reply: 2)
894. Plasmid with two different origins? - (reply: 1)
895. Chromosomal Gene Deletion - Gene Deletion by Double Crossing Over in Pseudomonas (reply: 6)
896. Do I need Gradient Thermal Cycler? - (reply: 20)
897. PGK-NEO whole sequence - (reply: 2)
898. primer tm with mismatch? - (reply: 6)
899. cDNA quantification for PCR - (reply: 5)
900. electroporation of agrobacterium - (reply: 1)

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