3451. Preserve PCR mix - (reply: 3)
3452. extract genomic DNA from plasma samples - (reply: 4)
3453. plasmid contamination - (reply: 2)
3454. PCR-RFLP - (reply: 2)
3455. calculation of transformation efficiency - (reply: 2)
3456. DNAse treatment in same step of RNA extraction - (reply: 5)
3457. primers design for mutagenesis of two nearby sites - mutations (reply: 2)
3458. Once more... cloning of a 13 kb fragment into pBR322 - (reply: 2)
3459. Asymmetric PCR - (reply: 2)
3460. Has anyone used this DNA sequencing product before? - (reply: 14)
3461. Re-use of columns for DNA-preparation - (reply: 2)
3462. Purify integrating PCR product before transforming yeast? - (reply: 1)
3463. What does the term "smear" mean in PCR ? - (reply: 3)
3464. ribosomal RNA contamination - (reply: 3)
3465. problems propagating pBluescript - reaaaaaally weird, help! (reply: 1)
3466. TAE, TBE vs TGE - what is the difference? (reply: 6)
3467. GFP or LacZ for quantification of promoter activity? - (reply: 4)
3468. RT-PCR Control Reactions - Need suggestions! - (reply: 1)
3469. expressing a gene in a cell HELP - (reply: 1)
3470. Mutagenesis to make 45bp deletion - (reply: 1)
3471. Troubleshooting PCR smear - Urgent,Need help! (reply: 4)
3472. Homology of plasmid sequence - Is Origin of relication sequence lifted from the bacterial genome? (reply: 2)
3473. Reamplifying my PCR product - (reply: 7)
3474. Trouble PCRing a long (7.5 kb) fragment - (reply: 6)
3475. [urgent help] Band missing in PCR - (reply: 5)
3476. DNase I Amplification grade and DNase I for RNA treatment - (reply: 1)
3477. How long DNA and siRNA stay in transfected cells - (reply: 2)
3478. URGENT-Selecting for transformed bacteria cells - (reply: 1)
3479. help with oligonucleotide precipitation assay - (reply: 1)
3480. why HEK293 or HEK293T is used to produce retrovirus? - retrovirus problem! (reply: 4)

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