4531. Cloning and PCR Problems - (reply: 1)
4532. Single cut vs uncut DNA - Mol. Biol. (reply: 1)
4533. DNA contamination in RNA extracted with RNeasy kit - (reply: 3)
4534. extract RNA from frozen cells - (reply: 6)
4535. Which buffers for storing of RNA and DNA? - (reply: 6)
4536. digestion problem - incomplete digestion of transformed plasmid (reply: 1)
4537. ds-RNA - DNA instead of dsRNA (reply: 1)
4538. rt-PCR false postitives - (reply: 2)
4539. why tanscription and translation coupled...... - (reply: 2)
4540. Problem with cloning - can't clone a 3.2kb fragment (reply: 5)
4541. Growing large plasmid (20kb) in e.coli - Low plasmid yield from large construct (reply: 7)
4542. Introduce ribosomal binding site (RBS) in pET?? - (reply: 1)
4543. problem with generation of restriction digestion site - (reply: 4)
4544. GATEWAY-CLONING - Experience with GATEWAY and exchange of clones (reply: 5)
4545. RNA isolation - combination of Trizol & colum-based kit (reply: 6)
4546. Expression Vector Construct - sequence entire insert? (reply: 3)
4547. Interesting gene expression in a stable transfection - (reply: 2)
4548. overlap extension and clone gene into pET -15b - a question?? (reply: 3)
4549. Identification of the cut and uncut plasmid on gel - (reply: 4)
4550. RT-PCR some questions - (reply: 2)
4551. Bad Primer - (reply: 2)
4552. DNA quantitation - why use a quartz cuvette? (reply: 2)
4553. Dual-promoter plasmids? - Trying to transcribe sense and antisense strands.. (reply: 1)
4554. Cloning of sequence with PCR added sites - BstBI and BclI (reply: 2)
4555. Blatting to human genome - (reply: 2)
4556. Strange results after transformation - (reply: 4)
4557. picking up colony - (reply: 2)
4558. PCR problems - (reply: 3)
4559. long range PCR problem - unable to amplify 12 kb fragment (reply: 4)
4560. Measuring NFkB activation - (reply: 2)

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