3001. Amplification of phage library - (reply: 3)
3002. Ligation Failure - 20 colonies tested with no results. Need advice (reply: 2)
3003. Endo-free DNA Preps, When? - For transfection?? For vaccines? (reply: 3)
3004. ligation only concatenate and not circularize - (reply: 2)
3005. ASK FOR HELP: why I can not get total RNA from human liver - (reply: 5)
3006. cDNA synthesis ASAP - gene expression (reply: 3)
3007. Off-target effects RNAi - Role of sense-strand (reply: 2)
3008. Western blotting trouble shooting - Protein transfer (reply: 4)
3009. Any one can tell me what happens to my RT-PCR? - Urgent !RT-PCR really kills me! (reply: 5)
3010. Problem with DNA extraction from plants - CTAB solution and nanodrop results (reply: 3)
3011. Cloning questions - (reply: 2)
3012. EMSA problems! - (reply: 5)
3013. How to check if reverse transcription went OK and there is cDNA - (reply: 5)
3014. cDNA tailind - (reply: 3)
3015. tRNA expression and purification - (reply: 1)
3016. enzyme that removes 5' overhangs - (reply: 1)
3017. DNA purification for transfection - someone have a protocol??? (reply: 1)
3018. Recombination pains in E.coli - (reply: 8)
3019. rpoD sequencing - (reply: 2)
3020. Transformation problem. Help me! - (reply: 6)
3021. PFGE - ?contamination - Contamination problem?! (reply: 3)
3022. restriction digestion of PCR product? - (reply: 10)
3023. phenol extraction problem - (reply: 5)
3024. northern blot probes - (reply: 1)
3025. Stuborn band after digestion of plasmid DNA - (reply: 4)
3026. isolation of plasmid - alkaline hydrolysis method (reply: 3)
3027. Induction of SAR - Resistant gene induction by salicylic acid (reply: 1)
3028. Taq polymerase vs HotstartTaq polymerase - (reply: 4)
3029. anyone got any idea how to make northern dot blots work? - I think it might be a probe problem (reply: 2)
3030. interactin between alkaline phosphatase buffer and PNK buffer. - help please (reply: 1)

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