3031. Best way to precipitate/concentrate genomic DNA? - (reply: 4)
3032. Why inducing competency in E. coli using calcium chloride requires ice - (reply: 2)
3033. sodium borate acid for DNA electrophoresis? - It's supposed to be much faster. Have you tried it? (reply: 13)
3034. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
3035. which voltage for which agarose concentration? - assuming same size of gel, how should voltage be adapted to agarose concentratio (reply: 3)
3036. Site-directed Mutagenesis - (reply: 22)
3037. partial proteolysis by trypsin - (reply: 2)
3038. methods to check orientation and frame in pQE30 - (reply: 6)
3039. Why ultra low freezer - (reply: 1)
3040. RNA extraction from whole blood using TRIzol - (reply: 1)
3041. pfx50 and pfuII - (reply: 2)
3042. BAC digestion - (reply: 8)
3043. cDNA tailing without terminal transferase - (reply: 1)
3044. endotoxin free plasmid preparation - kits comparable? (reply: 2)
3045. stupid RT-PCR - negative control suddenly positive?? (reply: 3)
3046. Question on Ethidium Bromide Gels - (reply: 2)
3047. Cant get desired fragment from plasmid - confused!!!!!!!!!!! (reply: 3)
3048. Sequencing - (reply: 5)
3049. cDNA synthesis primer - (reply: 3)
3050. Amplification of phage library - (reply: 3)
3051. Ligation Failure - 20 colonies tested with no results. Need advice (reply: 2)
3052. Endo-free DNA Preps, When? - For transfection?? For vaccines? (reply: 3)
3053. ligation only concatenate and not circularize - (reply: 2)
3054. ASK FOR HELP: why I can not get total RNA from human liver - (reply: 5)
3055. cDNA synthesis ASAP - gene expression (reply: 3)
3056. Off-target effects RNAi - Role of sense-strand (reply: 2)
3057. Western blotting trouble shooting - Protein transfer (reply: 4)
3058. Any one can tell me what happens to my RT-PCR? - Urgent !RT-PCR really kills me! (reply: 5)
3059. Problem with DNA extraction from plants - CTAB solution and nanodrop results (reply: 3)
3060. Cloning questions - (reply: 2)

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