2251. QuickChange primers self-made - (reply: 4)
2252. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
2253. running DNA on gel - (reply: 2)
2254. Tn10 based Transposone mutagenesis - pLBT::mini-Tn10:lac:kan (reply: 1)
2255. how to get specific amplfication of a gene that is extremely similar to another - (reply: 7)
2256. How to select candidate TFs for future study from the prediction results? - (reply: 4)
2257. cloning/restriction analyses MAJOR PROBLEM - (reply: 2)
2258. protein concentration - bradford method (reply: 9)
2259. PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
2260. K to R mutation for kinase activity change - (reply: 1)
2261. Genomic DNA cutting - (reply: 12)
2262. PCR for checking transfection - (reply: 6)
2263. Non specific amplification - How to check if the primers give non specific amplification or not (reply: 4)
2264. 3' bioninylated oligos for EMSA - (reply: 2)
2265. digoxygenin-labelled primer - (reply: 3)
2266. transfection - dna chloroform (reply: 1)
2267. gene delivery lipofectamine - lipoplexes acetone (reply: 4)
2268. Agarose Gel Electrophoresis - Troubleshoot (reply: 12)
2269. DNA conc for PCR - (reply: 1)
2270. Cloning 5kb into PBlueScript (2.9kb)? - (reply: 5)
2271. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
2272. enygmatic restriction by BamH1 - (reply: 8)
2273. How to determine 3' end sequence of bacterial gene - (reply: 6)
2274. Origin of replication - more, better ? (reply: 6)
2275. about the lentivector production - (reply: 3)
2276. Old PCR publication - help! - (reply: 2)
2277. C group Plasmids? - (reply: 2)
2278. freezing of primers - (reply: 2)
2279. Primers with RE sites - (reply: 2)
2280. Phusion kit altering my restriction sites only - (reply: 5)

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