3541. Impact of temperature and time increment during PCR - (reply: 3)
3542. Digestion Problem - Cloning (reply: 5)
3543. Multiplex Primers - Primer primer interaction (reply: 1)
3544. PRoduct lenght determination - product length determination (reply: 2)
3545. Agarose gel - DEPC-induced fragility? - RNA gels (reply: 2)
3546. pCMV-CD19 plasmid map - (reply: 2)
3547. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
3548. inconsistent PCR results - (reply: 2)
3549. topo XL cloning - (reply: 5)
3550. PCR smear in samples and negative control - (reply: 5)
3551. Protein imputities in extracted genomic DNA - (reply: 1)
3552. Is it necessary to treat total RNA with DNAse I when isolating mRNA? - (reply: 16)
3553. Problem in RNA isolation, degraded, even though I pay extra attention to prevent - (reply: 14)
3554. pcr sequencing - (reply: 2)
3555. DNA extraction from oilseed plants - (reply: 3)
3556. Exon intron boundaries - where to check those (reply: 3)
3557. RT-PCR of an intron - possible plateau effect? (reply: 2)
3558. Do you have any problem with QIAGEN Plasmid Maxi kit - (reply: 5)
3559. SDS-PAGE Staining - (reply: 5)
3560. Atb resistances - (reply: 4)
3561. Guys I need help please - (reply: 6)
3562. blunt end ligation - Help (reply: 11)
3563. Cellular RNA extraction - Sequence-selectivity - (reply: 2)
3564. phenol/chloroform of purified PCR product after RE digestion - what are we trying to purify? (reply: 4)
3565. biotinylated oligos - (reply: 6)
3566. PCR and topo problem - (reply: 3)
3567. help with background in northern blot - (reply: 1)
3568. Limit od detection and quantification - RT-PCR (reply: 1)
3569. RT-PCR primer design at 3'-UTR or ORF? - (reply: 2)
3570. Nhe1 Restriction digestion - (reply: 3)

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