4621. DNA yield from Frozen blood - DNA extraction from frozen humna blood (reply: 1)
4622. Gel extraction and elution buffer - (reply: 4)
4623. subcloning of PCR product, please help! - subcloning problems (reply: 12)
4624. cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
4625. Colony lift, help!!! - (reply: 1)
4626. Restriction site search - (reply: 7)
4627. factors contribute to the ligation failure.. - (reply: 1)
4628. 5.8S rRNA gene - help (reply: 2)
4629. maximum [EDTA] in restriction digests - (reply: 2)
4630. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
4631. RNA Quantification by spectrometer - 260 nm vs 230 nm (reply: 2)
4632. pGBKT7 miniprep problem - (reply: 5)
4633. DNA contamination in RNA extraction - (reply: 2)
4634. Double insert ligation? - (reply: 2)
4635. How long does it take E. coli to grow on plates at 30 degrees - transformation (reply: 4)
4636. DIG Southern Blots - Signal but very high background (reply: 1)
4637. phosphatase alkaline before cloning? - (reply: 3)
4638. Plasmid preparation problem using alkaline-lysis - (reply: 5)
4639. NAC (No Amplification Control) - (reply: 2)
4640. Recipe for anti-fade solution?? - (reply: 1)
4641. trouble ampilfy genomic DNA - degradation, contamination or..? (reply: 7)
4642. tRNA and digestion - (reply: 1)
4643. MMLVRT Temperature - (reply: 2)
4644. choice of in situ probes - (reply: 4)
4645. differents results in PCR and sequencing!! - (reply: 3)
4646. PCR contamination mystery - (reply: 2)
4647. convert the amount of DNA to number of diplod genome - (reply: 2)
4648. Does residual ethanol in DNA inhibit PCR? - ? (reply: 1)
4649. Problems with restriction enzymes - (reply: 4)
4650. How to get sharp band on agarose gel? - (reply: 7)

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