1741. Double-stranded oligo ligation - (reply: 13)
1742. Help me for DNA concentration for PCR - (reply: 4)
1743. Molar Ratio of Inser to Vector - (reply: 3)
1744. restriction digestion - (reply: 5)
1745. Gel purification of DNA bands-any alternates? - (reply: 4)
1746. Triton X in PCR - (reply: 7)
1747. PCR unspecific amplification - (reply: 10)
1748. AmpliTaq Gold - high fidelity polymerase? - (reply: 2)
1749. Digested DNA cleaning - My DNA is in dh2o and I need it to be in TE for the phenol/chloroform (reply: 6)
1750. sequencer - sequencer problem kit ABI 4336935 (reply: 1)
1751. What type of PCR to identify a plasmid insert? - (reply: 4)
1752. use of FLAG Ab - Immunofluorescence (reply: 8)
1753. Adenoviral Transfection - cells to check transfection efficiency (reply: 4)
1754. Buffer QBT - (reply: 2)
1755. ssDNA with Strandase Kit - (reply: 2)
1756. I have a challenge problem - Plasmid extraction (reply: 1)
1757. Howto: Maximum possible yield from a PCR? - What to optimize: Mgcl/primers/Polymerase/enhancers? (reply: 4)
1758. Will ATP inhibit Reverse Transcription? - (reply: 2)
1759. How much DNA in PCR? - (reply: 6)
1760. Different sequencing result of PCR products from the same cDNA - (reply: 2)
1761. mutation in a gene that is not expressing a protein - (reply: 4)
1762. competent cells transform 12 kb plasmid,urgent - (reply: 8)
1763. Help with Phenol/Chloroform extraction - (reply: 2)
1764. PCR problems - Fungal DNA (reply: 6)
1765. PCR contamination that comes and goes! - (reply: 5)
1766. Vesicle isolation - somebody know a protocol for that? (reply: 3)
1767. RNA extraction from pancreatic islets - (reply: 1)
1768. Screening stable clones - (reply: 2)
1769. question about Northern-blotting probe design - (reply: 3)
1770. Altering Maxiprep procedure to prepicpitate with ethanol instead of isoproponal - (reply: 6)

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