3091. How to measure biotinylated DNA? - (reply: 4)
3092. Northern hybridization - plz help (reply: 2)
3093. Fusion/overlap PCR troubleshooting - (reply: 3)
3094. Screening a Gene Library - I want to use colony PCR, but How (reply: 4)
3095. Question about Southern blotting - (reply: 4)
3096. how could one avoid amplification of a very high non-specific band from PCR - (reply: 3)
3097. methylated plasmid - (reply: 3)
3098. Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
3099. chemiluminescence detection. - (reply: 2)
3100. Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
3101. Protein dosage after sucrose gradient? - (reply: 2)
3102. NdeI double digestion - (reply: 3)
3103. Primer for 5' RACE - (reply: 2)
3104. Stick T7 on custom primer for sequencing? - (reply: 2)
3105. Any one who have this article? - (reply: 2)
3106. DNA extraction method from oil or fat sample - any standard procedures or commercial kits that allow isolated the total DNA fro (reply: 3)
3107. RNA isolation from Fungus - (reply: 1)
3108. Cultivate bacteriophage and isolate it's DNA from the plaque - (reply: 1)
3109. RNA polymerase? - (reply: 5)
3110. I don't know if I have RNA pellet or not - There are very little amount of RNA, Is there anything I can see to see the pell (reply: 3)
3111. Problem of Smear in PCR - Getting Smear in negetive control (reply: 4)
3112. Plasmid Purification - Any tricks to increase yield? What could I be doing wrong? (reply: 2)
3113. SMEAR IN POSITIVE CONTROL IN LONG PCR - (reply: 3)
3114. No colonies when transforming BL21 coli w/ chloramphenicol selection - (reply: 4)
3115. pGL3 transfection and assay - tips for improvement? - improve on low luciferase values?? (reply: 1)
3116. TB1 Competenet Cell - (reply: 2)
3117. DNA shearing methods - (reply: 4)
3118. Ratio of phenol:chloroform in RNA extraction - 1:1 or 5:1? (reply: 2)
3119. What’s the difference between ethanol and isopropanol precipitation? - (reply: 3)
3120. GC rich Exon 1 in ATP2A2 gene - Anyone heard of betaine? (reply: 3)

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