451. Current Protocol by John Wiley & Son - limited free access from invitrogen (reply: 1)
452. How to make my protein Soluble? - Bacterial system it sucksss ? (reply: 1)
453. cutting out EGFP gene from pEGFP-N1 vector - what does dam- mean? (reply: 1)
454. excessive ampicillin in L.B/Amp plates - (reply: 4)
455. false positives in PCR due to environmental contamination? - (reply: 7)
456. Primer design - How to - am a novice ? (reply: 6)
457. In Fusion cloning - reliable? (reply: 5)
458. request for agrobacterium mini prep protocol,not kit - (reply: 1)
459. PCR product size - (reply: 1)
460. how to convert ug to uL isolate of RNA? - (reply: 3)
461. is there simple method to know success first strand cDNA synthesis? - (reply: 1)
462. Cloning - False positive results - HELP!! (reply: 4)
463. Success with Invitrogen's pLenti6/v5-DEST? - (reply: 6)
464. Direct PCR from Gram positive bacteria - Is it possible? (reply: 3)
465. Genome walking - Urgent help! - Genome walking (reply: 3)
466. Where dose these unknown colonies come from? - (reply: 2)
467. Adding 3' A-overhangs for TA cloning - (reply: 4)
468. Sequencing contamination? - (reply: 2)
469. arms PCR problem - (reply: 2)
470. InFusion - (reply: 2)
471. mRNA purificaton - mRNA extraction from prokaryotes (reply: 1)
472. storing pcr samples - (reply: 19)
473. HELP. Mistakenly added 70% EtOH not 100% in PPT - (reply: 7)
474. Cold SSCP - Why we use Cold SSCP? (reply: 1)
475. what is the relationship between basepair and nano gram? - (reply: 2)
476. Problems with recombination BJ5183 - (reply: 1)
477. HEK293 for Adenovirus propagation - 293T, QB1 what's the difference? (reply: 1)
478. Site directed mutagenesis - several different multiple mutations (reply: 1)
479. Ask about linker ligation - (reply: 6)
480. how much agarose to use - (reply: 5)

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