3661. RNA-gel; how much RNA can I use? - (reply: 3)
3662. Smear after PCR - No band but smear were found from my samples (reply: 3)
3663. Where to find list of plasmid copy numbers? - I need to know approximate copy number before I choose vector (reply: 5)
3664. Buffer for amonium sulphate precipitation - (reply: 1)
3665. DNA migrates faster the longer you run a gel? - (reply: 4)
3666. Problem amplifying DNA from tissue - (reply: 4)
3667. Ligation of big vs small - (reply: 3)
3668. Question: Ligating and Cutting at the same time - (reply: 2)
3669. RNA isolation from tissues: RNA pellet is yellow-brown? - Why this? (reply: 2)
3670. Western Blot Hela Cos7 - Show nearly identical genes of two related organisms same expression (reply: 4)
3671. Extracting DNA from serum - (reply: 6)
3672. RNA isolation: ethonal wash step - Do you resuspend the RNA pellet by pipetting? (reply: 4)
3673. How do YOU precipitate your RNA? - (reply: 2)
3674. up-regulation of a gene - how long does it take for mRNA - are there general rules or time intervals known (reply: 4)
3675. KpnI Ligation Mystery - Bidirectional Cloning Working Unidirectionally (reply: 5)
3676. digestions sometimes working,sometimes not! - (reply: 3)
3677. Precipitation of 10X TBE - (reply: 18)
3678. high GC content in PCR? - (reply: 2)
3679. partial digestion problem - (reply: 8)
3680. RT PCR - (reply: 1)
3681. dsDNA quantitation via Realtime-Cycler and SybrGreenI - (reply: 1)
3682. suggestions? - (reply: 1)
3683. How to accurately quantify plasmid DNA in solution within impurity of RNA? - (reply: 3)
3684. Gel elution of a band - (reply: 4)
3685. help with buffer 5.1 for RNA extraction - (reply: 6)
3686. PCR internal control - how does it work? (reply: 1)
3687. Primer design for environmental samples - Align sequences first? (reply: 3)
3688. Mix Pfu and Taq - whether it can be done (reply: 5)
3689. advices - (reply: 4)
3690. False positive for PCR screening of insert - (reply: 1)

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