1531. ISHH Probe Design - (reply: 2)
1532. Circular ligation product migration on gel? - (reply: 1)
1533. how do i do flow cytometry for adherent cells? - (reply: 5)
1534. adenovirus purification - using CsCl (reply: 2)
1535. RNA gel electrophoresis - any recommendations? - (reply: 12)
1536. Ligation and transformation problem - (reply: 4)
1537. concentration of vector in ligation reaction - (reply: 4)
1538. non-UV measurement of DNA - (reply: 2)
1539. Trouble with viral vector --> too many colonies in control plate - Trouble with viral vector --> too many colonies in control plate (reply: 6)
1540. Why do I got 3 bands of RNA gel? - (reply: 3)
1541. In situ hybridization zebrafish embryo prep - paraformaldehyde damage embryos over time??? (reply: 2)
1542. Why the ligation does not work? - (reply: 7)
1543. how to extract plasmid spotted on paper? - (reply: 7)
1544. Restriction sites too close? - (reply: 2)
1545. Best way to inactivate Klenow fragment? - (reply: 4)
1546. puzzling RT question - (reply: 4)
1547. glycerol [c] for dna in gel - (reply: 5)
1548. Which vector would you choose for transient expression in CHO cells - (reply: 2)
1549. Miniprep from BL21(DE3) - (reply: 5)
1550. Ligationdifficulty - (reply: 5)
1551. Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
1552. cloning problem ....... - (reply: 4)
1553. Trouble with doing DNA Isolation and then running a PCR - (reply: 6)
1554. Aorta RNA isolation - (reply: 4)
1555. PCR: Can you get amplification if only one primer anneals? - Same size pcr product using 2 different primer sets (reply: 8)
1556. constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
1557. Total RNA isolation from mouse blastocysts - (reply: 1)
1558. RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
1559. 293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
1560. troubleshooting in RNA extraction with Trizol - (reply: 3)

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