4951. Sequential digestion with NheI and XhoI - Restrictive Digestion (reply: 3)
4952. Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
4953. CAT assay - WTF? (reply: 4)
4954. Gene expression - inducible Gene expression (reply: 1)
4955. problems purifying plasmid DNA after restriction digestion - (reply: 3)
4956. Agarose Gel Electrophoresis of plasmid DNA - Agarose Gel Electrophortesis of plasmid DNA (reply: 1)
4957. Question about cloning a gene coding an enzyme - (reply: 1)
4958. dsOligos annealing buffer - (reply: 2)
4959. Recombinant DNA - Problems with ligation (reply: 1)
4960. Leucine-rich repeats - (reply: 1)
4961. Real-time PCR problems - (reply: 3)
4962. Cloning rare splice variants - Molecular Biology (reply: 1)
4963. small sized restricted bands couldnt be visualized - restriction enzymes problems (reply: 22)
4964. Site Directed Mutagenesis Problem - (reply: 5)
4965. DNA purification kit for Microinjection - Anyone trying to purify fragements around 45 kb? (reply: 1)
4966. pQE30 Vs pET - Which is the better? (reply: 4)
4967. help with centrosome isolation - any advice much appreciated! thanks (reply: 1)
4968. triton x and np40 for nuclei isolation - (reply: 3)
4969. Sub-optimal PCR - SOS (reply: 1)
4970. Expression cloning system for protease expression - (reply: 2)
4971. Designing primers from amino acid sequence - cloning problem (reply: 3)
4972. RT-PCR for an intronless gene - (reply: 6)
4973. Large scale PCR to get milligram of DNA - (reply: 6)
4974. Cloning Large Fragments in a pool of DNA fragments - (reply: 1)
4975. making more plasmid - (reply: 2)
4976. Bioanalyser For RNA degradation, contradiction! - (reply: 1)
4977. Large insert cloning - (reply: 1)
4978. PCR woes - intermittent empty lanes (reply: 6)
4979. real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
4980. temarature sensitivity of RNA - mRNA is heat sensitive but (reply: 1)

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