1831. Isolation of total RNA from whole blood - How much Trizol do I need? (reply: 3)
1832. Ligation Difficulty - (reply: 2)
1833. PCR on PCR product - (reply: 5)
1834. recipe of 0.1M Glycine-NaOH buffer - (reply: 2)
1835. About housekeeping gene - (reply: 3)
1836. RT-PCR gene specific amplification problem - (reply: 1)
1837. Cloning short sequences - Does PCR can help clones upto 70 bps into a protein sequence (reply: 10)
1838. partial digestion of Tth111I - difficult??? (reply: 2)
1839. DNA contamination in microRNA sample - How to remove the genomic DNA from microRNA sample? (reply: 5)
1840. Self-circularization problem with pUC19 - (reply: 6)
1841. KOAZK rule - what is it??? (reply: 6)
1842. DNA isolation from Dendrobium - (reply: 7)
1843. information regarding PCR using very long primers - (reply: 4)
1844. RT-PCR: Band in no reverse transcriptase lane - (reply: 18)
1845. Restriction of pichia vector pPICZ alpha with Xho1 and Xba1 - (reply: 1)
1846. Non repeatability of RT-PCR with same cDNA - (reply: 3)
1847. Keep RT mix on ice for 4 hrs - RT-PCR (reply: 3)
1848. Making miniprep solutions for spin columns - Now that we can reuse the columns... (reply: 21)
1849. housekeeping genes - (reply: 1)
1850. whole gene synthesis for expression - (reply: 3)
1851. Need denature (94oc in 5 mintute) for agarose gel? - (reply: 7)
1852. RNA isolation stationary phase - (reply: 1)
1853. How to wash pellet before RNA isolation - (reply: 1)
1854. why need the "Data collection step" - (reply: 3)
1855. How to know there is cDNA? - (reply: 5)
1856. partial digestion of Tth111I - (reply: 1)
1857. agarose gel electrophoresis - problems in marker migration (reply: 14)
1858. Mulitplex problems - (reply: 3)
1859. Shock--nothing at all after washing membrane in northern blotting - (reply: 2)
1860. I really need an answer on PCR (16s rRNA), PLS PLS PLS! - (reply: 2)

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