4561. Gene expression comparison between different species(!) - (reply: 1)
4562. delta delta Ct method - Real time PCR (reply: 4)
4563. Is it ok to store a digested vector for ligation? - (reply: 4)
4564. Transforming with large AT rich inserts - (reply: 1)
4565. Isolation of HIV + DNA - (reply: 2)
4566. A tried and true cloning protocol is FAILING miserably! - Molecular biology (reply: 6)
4567. Reverse-transcriptase PCR troubleshoot HELP - (reply: 13)
4568. three fragments ligation problems - emergent help needed (reply: 2)
4569. reducing primer dimer - (reply: 1)
4570. RNA vs DNA purifucation - how are they separated from each other? (reply: 5)
4571. PCR troubleshooting - Primers (reply: 3)
4572. should we dilute the DNA (vector and insert), before ligation? - ligation problem (reply: 5)
4573. activity of T4 polynucleotide kinase - (reply: 1)
4574. restriction enzyme mapping - (reply: 1)
4575. No expression of 7kDa His-tagged protein - why? - Poor protein expression (reply: 9)
4576. Museum DNA - Extracting and PCRing ancient DNA (reply: 3)
4577. Northern blot - How do you do crosslinking - (reply: 6)
4578. PCR problem amplifying 4 kb DNA - (reply: 5)
4579. DNA extraction problems using alkaline lysis method - It stopped working! (reply: 3)
4580. How does plasmid DNA miniprep kit get rid of chromosome DNA? - (reply: 4)
4581. Calculating correct melting temperature for primers - (reply: 2)
4582. DNA Extraction Problem (No DNA, just a white salt) - (reply: 5)
4583. Clontech pEGFP-N1 and -C1 vectors - (reply: 4)
4584. Plasmid wash in E. coli? - How to promote the lost of a plasmid in coli (reply: 2)
4585. competent cells question - (reply: 3)
4586. How can I add sequences to DNA using PCR? - (reply: 1)
4587. anyone using TRIzol from invitrogen? - (reply: 3)
4588. How to attach a molecule to DNA - (reply: 2)
4589. ligation problem - (reply: 6)
4590. any effect if the plasmid got 3 gen resistance? - (reply: 1)

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