3811. What type of DNA does E.coli contain? - (reply: 2)
3812. over time ligation - (reply: 5)
3813. His-tag design in primer - DO i need filler. (reply: 4)
3814. DTT & RNase inhibitor in RT reaction - (reply: 6)
3815. cre recombinase - in cell lines (reply: 2)
3816. Troubleshooting ligation/transformation - (reply: 1)
3817. gene promoter - promoter luciferase (reply: 3)
3818. How to extract DNA of Bacillus subtilis efficiently? - (reply: 7)
3819. PstI restrictase (Invitrogen) - reaction buffer (reply: 1)
3820. Miniprep plasmid shows three bands - (reply: 1)
3821. ACE PCR - problems with PCR for angiotensin-converting enzyme (reply: 2)
3822. Amplify 4.5 kb pcr product without using special polymerase - (reply: 10)
3823. PCR master mix - (reply: 6)
3824. RECOMBINEERING - (reply: 1)
3825. SOS! asymmetric PCR, no PCR product - (reply: 2)
3826. Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
3827. degenerate PCR vs low stringency PCR - (reply: 1)
3828. how to mutant the gene on the genomic DNA? - (reply: 9)
3829. RT-PCR- help - Polyacrylmide Gel electrophrosis for PCR products (reply: 3)
3830. poly (dI-dC) - poly (dI-dC) role? (reply: 1)
3831. CsCl gradient - (reply: 1)
3832. No DNA on gel, but get PCR product - (reply: 2)
3833. What media would promote the best E.coli growth at a low cost for fermentation? - (reply: 18)
3834. handling cDNA - care in handling (reply: 1)
3835. explanation of miniprep of E. coli - miniprep of e.coli genomic dna? (reply: 1)
3836. Protocol for DNase I treatment of RNA - (reply: 4)
3837. PCR primer: 2 restriction sites? - (reply: 4)
3838. determine the molecular mechanisms for gene regulation - (reply: 1)
3839. Weird Band gel migration - Molecular biology (reply: 7)
3840. No PCR Product - Fungal Genomic PCR (reply: 9)

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