1621. glycerol [c] for dna in gel - (reply: 5)
1622. Which vector would you choose for transient expression in CHO cells - (reply: 2)
1623. Miniprep from BL21(DE3) - (reply: 5)
1624. Ligationdifficulty - (reply: 5)
1625. Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
1626. cloning problem ....... - (reply: 4)
1627. Trouble with doing DNA Isolation and then running a PCR - (reply: 6)
1628. Aorta RNA isolation - (reply: 4)
1629. PCR: Can you get amplification if only one primer anneals? - Same size pcr product using 2 different primer sets (reply: 8)
1630. constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
1631. Total RNA isolation from mouse blastocysts - (reply: 1)
1632. RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
1633. 293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
1634. troubleshooting in RNA extraction with Trizol - (reply: 3)
1635. E.coli Compotent Cell Storage and Plasmid Isolation - (reply: 3)
1636. three way ligation - three way ligation help (reply: 6)
1637. Southern blot help - (reply: 1)
1638. Closing up restriction sites - KpnI/XhoI site needs to get closed (reply: 2)
1639. COBAS Amplicor device - can u help me understand this.. (reply: 1)
1640. Non-isotopic EMSA-dirty uneven background - (reply: 7)
1641. phage cDNA library screening with PCR - (reply: 3)
1642. How can I send RNA samples for microarray application - general lab techniques (reply: 1)
1643. Construction of isogenic mutants help - Construction of isogenic mutants (reply: 6)
1644. can i use distilled water instead of double distilled? - RBC's lysis step (reply: 1)
1645. PCR help! - urgent (reply: 5)
1646. Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
1647. PCR trouble: about the annealing temperature - (reply: 4)
1648. How much ligase per ug of DNA? - (reply: 6)
1649. Gel purification of plasmid after each of these steps? - (reply: 1)
1650. Plasmid pSPL3 - Looking for plasmid pSPL3 (reply: 2)

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