2161. primer Tm - (reply: 10)
2162. PCR product 1200bp - (reply: 3)
2163. Stable Clone establishment using Hygromycin or Puromycin vectors - (reply: 3)
2164. A 270 for RNA - (reply: 1)
2165. Homologous recombination - (reply: 1)
2166. PCR cloning strategy - (reply: 4)
2167. Site-Directed Mutagenesis (megaprimer) of internal sequence - (reply: 5)
2168. in vitro transcription - transcription of long transcripts (reply: 1)
2169. problems with yields from qiagen spin mini prep kit - (reply: 21)
2170. avicel medium for RNA extraction - crushing of avicel (reply: 1)
2171. smear in degenerate PCR - (reply: 3)
2172. dUTP s - Explanation ! (reply: 1)
2173. strange sequencing / cloning problem with AvrII-site - (reply: 2)
2174. What is the function of NADPH in the reaction system? - (reply: 2)
2175. Proteinase K - How to use it? (reply: 2)
2176. Single Codon Deletion - SOE PCR - (reply: 3)
2177. QuickChange primers self-made - (reply: 4)
2178. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
2179. running DNA on gel - (reply: 2)
2180. Tn10 based Transposone mutagenesis - pLBT::mini-Tn10:lac:kan (reply: 1)
2181. how to get specific amplfication of a gene that is extremely similar to another - (reply: 7)
2182. How to select candidate TFs for future study from the prediction results? - (reply: 4)
2183. cloning/restriction analyses MAJOR PROBLEM - (reply: 2)
2184. protein concentration - bradford method (reply: 9)
2185. PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
2186. K to R mutation for kinase activity change - (reply: 1)
2187. Genomic DNA cutting - (reply: 12)
2188. PCR for checking transfection - (reply: 6)
2189. Non specific amplification - How to check if the primers give non specific amplification or not (reply: 4)
2190. 3' bioninylated oligos for EMSA - (reply: 2)

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