1141. How to determine DNA sequence is from male or female? - (reply: 5)
1142. EMSA problem - (reply: 4)
1143. yeast colony PCR - How to improve yeast colony PCR (reply: 7)
1144. BLOOD storage and PCR problems! HELP - (reply: 1)
1145. Anyway to lyse bacteria but do not lyse WBC? - in human blood samples (reply: 2)
1146. fibroblast marker genes - fibroblast marker genes (reply: 1)
1147. stop-transfer mechanism - (reply: 1)
1148. EGFP constructed in T-vector can express green fluorescence? - SOS (reply: 6)
1149. Sprinting fragments? - (reply: 1)
1150. pcr problems - pcr to solve (reply: 2)
1151. Calculating protein size - how to calculate the protein size when cloned in pet vector (reply: 1)
1152. Thermostable Reverse Transcriptase for RT-PCR - (reply: 3)
1153. figure out the size of amplicon using known primer - (reply: 3)
1154. Blunt end cloning question - (reply: 1)
1155. Molecular Cloning: A Laboratory Manual (3-Volume Set) - buy now or later? (reply: 4)
1156. Primer concentration... - (reply: 5)
1157. Separation of DNA bands of sizes 8.86 kb and 9.5 kb - (reply: 3)
1158. Strange background in lanes using Pierce Lightshift EMSA - (reply: 1)
1159. from Trizol to phenol/chloroform for DNA extraction - (reply: 1)
1160. How to amplify PCR product - wonder why using linear fragment as template, PCR result was always sm (reply: 1)
1161. Ratio of labelled vs. unlabelled species in "uptake assay" - Whether to use a fixed amount or a fixed concentration of label (reply: 1)
1162. colocalization of mitochondrion and GFP fusion - (reply: 2)
1163. Question about ligation! - Ligation of 6kb insert in pGEM T-vector (reply: 1)
1164. i'm puzzled by Taq polymerase - Taq adds A tail (reply: 4)
1165. RNA storage for microarray study - (reply: 1)
1166. Long PCR primers for epitope tagging - where to order? (reply: 3)
1167. Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
1168. Why we don’t use large amount of sample to extract DNA by chelex method? - (reply: 2)
1169. function of isoamyle alcohol in organic method of DNA extraction - (reply: 1)
1170. PCR screening for recombinant clones - (reply: 1)

© 1999-2008 Protocol Online, All rights reserved.