1441. How to identify transcription factors binding to an specific DNA sequence? - (reply: 5)
1442. Southern Blost Kit HELP! - (reply: 1)
1443. Bright wells! - (reply: 3)
1444. Optimum annealing temperature - Tm calculator (reply: 12)
1445. The top of PAGE gel is not polymerizing - (reply: 6)
1446. RNA Isolation from hydatidiform mole - (reply: 2)
1447. Plasmid Cleanup - Advice on how to further clean up plasmid created by alkaline lysis an (reply: 4)
1448. DNA extraction - starting from mouse ear tissue (reply: 1)
1449. Problem with RNA isolation/extraction of plasma - When RNA isolation form supernatant it's fine, not from plasma, us (reply: 1)
1450. Problems with Southern Blot - Probe labeling - (reply: 1)
1451. Problems experienced from adding 3xHA epitope to the C- terminus of some interes - Problems experienced from adding 3xHA epitope to the C- terminus of so (reply: 2)
1452. sticky end to blunt end? - cloning (reply: 3)
1453. advice on DNA extraxtion: boiling method - (reply: 5)
1454. Could competent cell be stored in liquid nitrogen? - (reply: 7)
1455. paper request - (reply: 2)
1456. PCR amplification of region with many ATT repeats - (reply: 1)
1457. Unable to ligate two PCR products - (reply: 4)
1458. PCR primers artifact - (reply: 2)
1459. not getting transformants - (reply: 12)
1460. cloning faint bands - (reply: 5)
1461. Purification of digested PCR product (a smear fr 200bp to 3kb) - (reply: 1)
1462. pGL3 Luciferase assay - need experimental set up help (reply: 1)
1463. See small size difference on gel? - (reply: 2)
1464. Is this clone the correct one? - Restriction digestion doubt (reply: 1)
1465. weak RT-PCR product - (reply: 2)
1466. ok to use Trizol w/ chloroform:isoamyl? - (reply: 2)
1467. SacII digestion - (reply: 1)
1468. Will restrictases cut this close? - (reply: 3)
1469. fillin - bluntend (reply: 4)
1470. extractin RNA from cells grown on Matrigel - (reply: 1)

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