2941. isolate RNA from small size sample - (reply: 1)
2942. Fluorscein- Fluorimager how to - (reply: 1)
2943. PCR amplification of Community DNA - (reply: 6)
2944. thermal sensitivity? - (reply: 1)
2945. library titering and amplification - (reply: 1)
2946. elute cDNA on filter paper - (reply: 3)
2947. Site-directed Mutagenesis - (reply: 3)
2948. protein leftover in DNA - how to get rid of it? (reply: 4)
2949. Whether Mg2+ can be released from the Reverse Transcriptase after Its Inactivati - Urgent (reply: 3)
2950. Vector: pIRES - (reply: 4)
2951. Concentration of cDNA after reverse transcription--no spec? - (reply: 2)
2952. nested PCR: purification? - (reply: 1)
2953. PCR problems with 1,7kb fragment - (reply: 8)
2954. RNA isolation from trypsinized cells - (reply: 4)
2955. cDNA -mRNA hybrid - (reply: 4)
2956. Help needed - What is the influence of high degree of cholesterol in liposome composition (1/3 (reply: 1)
2957. Best way to precipitate/concentrate genomic DNA? - (reply: 4)
2958. Why inducing competency in E. coli using calcium chloride requires ice - (reply: 2)
2959. sodium borate acid for DNA electrophoresis? - It's supposed to be much faster. Have you tried it? (reply: 13)
2960. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
2961. which voltage for which agarose concentration? - assuming same size of gel, how should voltage be adapted to agarose concentratio (reply: 3)
2962. Site-directed Mutagenesis - (reply: 22)
2963. partial proteolysis by trypsin - (reply: 2)
2964. methods to check orientation and frame in pQE30 - (reply: 6)
2965. Why ultra low freezer - (reply: 1)
2966. RNA extraction from whole blood using TRIzol - (reply: 1)
2967. pfx50 and pfuII - (reply: 2)
2968. BAC digestion - (reply: 8)
2969. cDNA tailing without terminal transferase - (reply: 1)
2970. endotoxin free plasmid preparation - kits comparable? (reply: 2)

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