2971. elute cDNA on filter paper - (reply: 3)
2972. Site-directed Mutagenesis - (reply: 3)
2973. protein leftover in DNA - how to get rid of it? (reply: 4)
2974. Whether Mg2+ can be released from the Reverse Transcriptase after Its Inactivati - Urgent (reply: 3)
2975. Vector: pIRES - (reply: 4)
2976. Concentration of cDNA after reverse transcription--no spec? - (reply: 2)
2977. nested PCR: purification? - (reply: 1)
2978. PCR problems with 1,7kb fragment - (reply: 8)
2979. RNA isolation from trypsinized cells - (reply: 4)
2980. cDNA -mRNA hybrid - (reply: 4)
2981. Help needed - What is the influence of high degree of cholesterol in liposome composition (1/3 (reply: 1)
2982. Best way to precipitate/concentrate genomic DNA? - (reply: 4)
2983. Why inducing competency in E. coli using calcium chloride requires ice - (reply: 2)
2984. sodium borate acid for DNA electrophoresis? - It's supposed to be much faster. Have you tried it? (reply: 13)
2985. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
2986. which voltage for which agarose concentration? - assuming same size of gel, how should voltage be adapted to agarose concentratio (reply: 3)
2987. Site-directed Mutagenesis - (reply: 22)
2988. partial proteolysis by trypsin - (reply: 2)
2989. methods to check orientation and frame in pQE30 - (reply: 6)
2990. Why ultra low freezer - (reply: 1)
2991. RNA extraction from whole blood using TRIzol - (reply: 1)
2992. pfx50 and pfuII - (reply: 2)
2993. BAC digestion - (reply: 8)
2994. cDNA tailing without terminal transferase - (reply: 1)
2995. endotoxin free plasmid preparation - kits comparable? (reply: 2)
2996. stupid RT-PCR - negative control suddenly positive?? (reply: 3)
2997. Question on Ethidium Bromide Gels - (reply: 2)
2998. Cant get desired fragment from plasmid - confused!!!!!!!!!!! (reply: 3)
2999. Sequencing - (reply: 5)
3000. cDNA synthesis primer - (reply: 3)

© 1999-2008 Protocol Online, All rights reserved.