1201. double digestion of transformed bacteria colony - (reply: 7)
1202. Number of DNA entering during transformation - How many molecules of DNA are taken up during transformation (reply: 2)
1203. sequencing not good after reading poly A tail..why? - (reply: 3)
1204. smear when cutting plasmids previously checked - (reply: 4)
1205. storage of ammonium sulphate purified antibodies - (reply: 1)
1206. Competent cells not growing - (reply: 5)
1207. How to amplify 4.1Kb gene from genomic DNA? - (reply: 15)
1208. cDNA library - Looking for the best company to order from (reply: 2)
1209. Transfection - Something wrong with transfection? (reply: 4)
1210. What is the use of tRNA? - (reply: 2)
1211. cDNA cloning using Taq/pfu polymerase - (reply: 2)
1212. Non-specific Real time PCR signals - (reply: 1)
1213. Mutagenesis PCR transforming into BL21(DE3) - (reply: 2)
1214. yeast transformation failed after doing it many times - (reply: 5)
1215. PCR amplificaiton isnt working - (reply: 12)
1216. lentiviral vector cloning problem - (reply: 1)
1217. Transparent pellet - (reply: 8)
1218. Problems on overlap extenstion PCR - (reply: 2)
1219. composition of Qiagen RDD buffer - (reply: 1)
1220. Using mRNA in cloning - (reply: 1)
1221. about heatshock transformation - (reply: 1)
1222. competent cell didn't grow in LB - (reply: 15)
1223. A-overhang in PCR product - (reply: 2)
1224. Cox-2 primer for PCR - method how to search good cDNA in Pubmed (reply: 1)
1225. How to purify 700bp pcr product? - (reply: 5)
1226. problems with E.coli heat shock transformations - (reply: 1)
1227. Problems in CaCl2 method ofr competent cells - (reply: 1)
1228. Northern Blot - can't detect my mRNA - (reply: 1)
1229. Bac recomb: cre recomb - (reply: 4)
1230. Protease / Phosphatase Inbibitor necessary ? - (reply: 4)

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