211. PCR troubleshooting - (reply: 10)
212. pET-16b vector in DH5alpha E. coli - (reply: 7)
213. TRIzol wasn't homogenized with sample? - (reply: 4)
214. RNA electrophoresis - different concentration of ethidium bromide between gel and buffer (reply: 2)
215. cytosolic b-catenin expression - (reply: 2)
216. DNAse inhibitors - (reply: 1)
217. how to optimize rt-pcr for gene expression analysis - (reply: 3)
218. ghost band in PCR? - (reply: 10)
219. What is my control when running a PCR reaction? - (reply: 2)
220. genomic library - use of restriction enzymes (reply: 10)
221. selective splicing? - selective splicing? (reply: 4)
222. Protein come from nowhere - we cannot find RNA (reply: 5)
223. How to synthesize your own oligo-dT primer - (reply: 4)
224. Arg-F lac E. coli - (reply: 1)
225. PCR Problem - 300bp skip in the middle (reply: 9)
226. Using pGEMT Vectors - (reply: 4)
227. A little more help with PCR! - (reply: 2)
228. wierd dna sequencing results - any clues? (reply: 4)
229. DNA laddering - apoptosis - any suggestion about the extraction procedure ? (reply: 1)
230. invitrogen platinum pfx taq - any comments? (reply: 6)
231. DNA Sequencing Problem - (reply: 6)
232. transformation of polyploid industrial Saccharomyces - (reply: 5)
233. Designing Primers - (reply: 1)
234. Basic question about setting up a PCR reaction - (reply: 4)
235. Phenol:chloroform:isoamyl pH 5 for DNA extraction? - Kinda worried, only have pH 8 but that should be fine right? (reply: 1)
236. basic basic question regarding pcr product storage - (reply: 2)
237. DNA pooling - (reply: 1)
238. Restriction Enzyme digestion - (reply: 4)
239. rolling circle amplification - (reply: 1)
240. Preparing bacterial protoplasts! - anyone has a good protocol? (reply: 3)

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