5281. How to determine PCR annealing temperature - (reply: 9)
5282. How to label a probe for Northern blotting - (reply: 5)
5283. How to find the transcription start site of an unknown gene? - (reply: 1)
5284. Double Digest problems - (reply: 2)
5285. Cloning Isoforms - (reply: 1)
5286. frameshift after ligation - point-mutation in restriction site (reply: 3)
5287. sometimes I get it sometimes I dont - My PCr does not work always (reply: 9)
5288. Using a big primer in PCR - (reply: 4)
5289. No colony came out on the selective plate - (reply: 2)
5290. allele-specific PCR - detection of SNPs by allele-specific amplification (reply: 4)
5291. Bad OD ratios from Plasmid Minipreps - (reply: 2)
5292. Isolation restr. fragments from agarose gel by Qiagen kit - cloning fungal genomic DNA (reply: 1)
5293. Rephosphorylation of SAP treated DNA fragment - (reply: 1)
5294. how to separate DNA of similar size - (reply: 5)
5295. use of direct current for electrophoresis - (reply: 1)
5296. No PCR product - Environmental samples (reply: 2)
5297. topo cloning - tet casette (reply: 2)
5298. Nucleic acid adherence to plastics - Nucleic acid adherence to plastics (reply: 2)
5299. Does somebody use AvrII - Help (reply: 4)
5300. RNA extraction - RNA from 20yr old frozen breast tissue (reply: 1)
5301. designing probes - identify phage-like sequences (reply: 1)
5302. How to remove PCR inhibitors from DNA - (reply: 7)
5303. cloning problem - cloning problem in GFP vector (reply: 4)
5304. question about UV melting experiments - asking for help with expt method (reply: 1)
5305. deionised formamide - deionised formamide (reply: 1)
5306. How to get rid of PCR primer dimer - (reply: 62)
5307. Problem cloning PCR product into double-cut vector - (reply: 3)
5308. Lyophlization - Mechanisms of Lyophlization (reply: 1)
5309. I forgot to pretreat my dialysis membrane ! - (reply: 1)
5310. RT-PCR - double-strand RNA denaturation (reply: 2)

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