4771. Cloning a large PCR product - trouble cloning large DNA (reply: 3)
4772. double sized PCR product from cloned cDNA - (reply: 6)
4773. Is kozak sequence necessary for cloning? - (reply: 4)
4774. mysterious 1.6kb band after transformation - (reply: 1)
4775. amount of DNA for clontech genome walker system - how much (reply: 2)
4776. problem with DNA extraction - can't get rid of protein phase (reply: 3)
4777. real-time pcr plasmid standards - (reply: 4)
4778. RT-PCR and RE cut site-will this work? - (reply: 3)
4779. DNA ladder for total RNA integrity test? - (reply: 1)
4780. ligation problem.. - (reply: 3)
4781. PCR of plasmid miniprep from Agrobacterium - Can't get PCR product out of plasmid miniprep (reply: 1)
4782. PCR parameters - (reply: 2)
4783. Both total RNA and mRNA in the same reaction? - (reply: 1)
4784. Is PNK treatment required for my insert? - Is PNK treatment required for my insert after i CI (reply: 2)
4785. phosphate group in DNA - (reply: 1)
4786. southern blot expert needed - How do you improve your southern? (reply: 4)
4787. DNA got degraded after klenow, CIP and Phenol extraction - (reply: 1)
4788. Unspecific bands after gel purification, why? - (reply: 7)
4789. cDNA-measuring? - (reply: 4)
4790. Plant gene expression in bacteria and yeast - (reply: 3)
4791. DNA extraction using RNAse (help needed) - (reply: 1)
4792. Unequal amplification efficiency of heat blocks - (reply: 4)
4793. DNA in 80mM NaOH - (reply: 2)
4794. CDNA clean up protocol - (reply: 2)
4795. DNA extraction including RNAse - (reply: 1)
4796. DNA binding by RE causes bigger band than substrate in gel? - Restriction digest of 500bp frag. with BsaXI (reply: 4)
4797. Whatman Filter Paper - Elution of plasmid DNA (reply: 1)
4798. problem with ligation with ClaI - Cloning (reply: 7)
4799. possible coding of stop codon? - (reply: 3)
4800. PCR off of Genomic DNA problems! - -why is my gene without introns showing up?- (reply: 3)

© 1999-2008 Protocol Online, All rights reserved.