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Store ligation reaction for repeat transformation? - (May/18/2005 )

Hi,
I have recently started cloning PCR products and have been having a few problems. One question I have that I can't find an answer to anywhere is whether a ligation reaction can be stored in the freezer and used again. If anyone out there can help I would be most appreciative.

-Sofie76-

sure
i had stored the ligation product at 4 or -20℃, and successfully ligated.

-knewman-

Thanks for the help,
It actually worked better this time than the first time!

-Sofie76-

you are wellcom

-knewman-