4711. phosphatase alkaline before cloning? - (reply: 3)
4712. Plasmid preparation problem using alkaline-lysis - (reply: 5)
4713. NAC (No Amplification Control) - (reply: 2)
4714. Recipe for anti-fade solution?? - (reply: 1)
4715. trouble ampilfy genomic DNA - degradation, contamination or..? (reply: 7)
4716. tRNA and digestion - (reply: 1)
4717. MMLVRT Temperature - (reply: 2)
4718. choice of in situ probes - (reply: 4)
4719. differents results in PCR and sequencing!! - (reply: 3)
4720. PCR contamination mystery - (reply: 2)
4721. convert the amount of DNA to number of diplod genome - (reply: 2)
4722. Does residual ethanol in DNA inhibit PCR? - ? (reply: 1)
4723. Problems with restriction enzymes - (reply: 4)
4724. How to get sharp band on agarose gel? - (reply: 7)
4725. Putting 2 plasmids in 1 e. coli cell - (reply: 4)
4726. How would you identify and clone quinine transporters for P. - (reply: 1)
4727. genes orientation - (reply: 3)
4728. Volatge problem - (reply: 1)
4729. ATP in ligation reaction - ligation efficiency (reply: 3)
4730. how to analyse the gene haplotypes - (reply: 1)
4731. Gel extraction problem - (reply: 15)
4732. Baseline and threshold adjustment - (reply: 1)
4733. Help needed in library construction of metagenome - (reply: 16)
4734. HEPA filter - and PCR contamination (reply: 1)
4735. antibiotics concentration for ligated plasmid - (reply: 3)
4736. circular vector propagation - How? (reply: 1)
4737. Trizol followed by column purification - (reply: 9)
4738. importance of directionality in cloning - (reply: 1)
4739. No colonies with site-directed mutagenic plasmids - (reply: 2)
4740. Problem with amplifying DNA from FTA cards - Is there a special trick involved? (reply: 1)

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