1261. PCR inhibitors in soil DNA - (reply: 6)
1262. Epitope tagging - Several basic questions (reply: 2)
1263. southern blot hybridization - DIG - (reply: 4)
1264. What is the best way to align multiple DNA sequences? - (reply: 2)
1265. Best DNA sequence analysis tool? - what is your favorite? (reply: 5)
1266. difference in sequencing results - (reply: 8)
1267. PLasmid extraction: good nanodrop reading but NOTHING on the gel! - (reply: 4)
1268. differential binding of dye to supercoiled and relaxed plasmid DNA - which dye and how? (reply: 5)
1269. EST vs. FL cDNA? - (reply: 6)
1270. 2 week old maxiprep DNA smearing - (reply: 4)
1271. Blunt or sticky ligation-Which is more efficient? - (reply: 3)
1272. Enzymatic reaction cleanups- Columns or Phenol-chloroform extraction? - (reply: 2)
1273. PCR of AT-rich DNA - (reply: 3)
1274. 2D-PAGE MARKER TROUBLE - (reply: 1)
1275. double digestion of transformed bacteria colony - (reply: 7)
1276. Number of DNA entering during transformation - How many molecules of DNA are taken up during transformation (reply: 2)
1277. sequencing not good after reading poly A tail..why? - (reply: 3)
1278. smear when cutting plasmids previously checked - (reply: 4)
1279. storage of ammonium sulphate purified antibodies - (reply: 1)
1280. Competent cells not growing - (reply: 5)
1281. How to amplify 4.1Kb gene from genomic DNA? - (reply: 15)
1282. cDNA library - Looking for the best company to order from (reply: 2)
1283. Transfection - Something wrong with transfection? (reply: 4)
1284. What is the use of tRNA? - (reply: 2)
1285. cDNA cloning using Taq/pfu polymerase - (reply: 2)
1286. Non-specific Real time PCR signals - (reply: 1)
1287. Mutagenesis PCR transforming into BL21(DE3) - (reply: 2)
1288. yeast transformation failed after doing it many times - (reply: 5)
1289. PCR amplificaiton isnt working - (reply: 12)
1290. lentiviral vector cloning problem - (reply: 1)

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