1381. How to make EGTA solution? - (reply: 2)
1382. How to sterilize mortar/pestle free of RNases - (reply: 5)
1383. Sequencing protocol ....? - (reply: 2)
1384. Question about QuikChange II mutagensis method - Can it merge plasmids? (reply: 3)
1385. problems with microsatellite sequences - (reply: 1)
1386. how to explain this strange amplification chart? - the Ct value comes to be very low because of the unusual amplification (reply: 1)
1387. Insert only ligating in backward direction - (reply: 2)
1388. PCR Troubleshooting - (reply: 2)
1389. Transformation problem with newly ligated plasmids - (reply: 1)
1390. cDNA - amonut to use (reply: 3)
1391. RNA-chip - (reply: 1)
1392. Sequence editing - (reply: 1)
1393. Formaldehyde Gel Electrophoresis on non-denatured/denatured RNA - No/Faint RNA bands after denatration (reply: 1)
1394. My dot blot hybridization doesn't work! - (reply: 21)
1395. EMSA probe: two bands in free probe lane - (reply: 1)
1396. Changing Valine to Leucine - (reply: 1)
1397. Nested PCR - (reply: 5)
1398. DNAse I - Degrading my DNA (reply: 2)
1399. Need help about my subcloning pET15b. - (reply: 1)
1400. recipe for SB buffer for electrophoresis - (reply: 4)
1401. i dont get colonies - (reply: 3)
1402. primer design for PCR cloning - (reply: 1)
1403. how to make a sucrose gradient? - (reply: 1)
1404. What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb? (reply: 6)
1405. in vitro synthesis of RNA using T7 - (reply: 2)
1406. annealing s.s. DNA - (reply: 2)
1407. deletion PCR? - I need help (reply: 1)
1408. Differences seen in regular and real time machine - (reply: 2)
1409. Eukaryotic recombinant protein expression - (reply: 3)
1410. your experience with long range PCR - (reply: 3)

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