2701. NF-kB EMSA - Different nuclear protein extraction method leads to opposite results (reply: 1)
2702. dna purification - (reply: 6)
2703. PCR problems, huge artifacts, no product - involves 16S primers (reply: 8)
2704. low 260/280 ratio: 1.4 - How can i improve quality?? (reply: 8)
2705. How to make electrocompetent cells without refrigerated centrifugue - (reply: 3)
2706. DNA prep from Serum - trying to isolate small fragments of DNA in human serum (reply: 1)
2707. TF-bindingsite in luciferase - (reply: 2)
2708. about RAPD PCR of plant genomes? - (reply: 4)
2709. transformation problem - (reply: 3)
2710. phenol/chloroform extraction.... - protocols for large/medium scale (reply: 5)
2711. studying apoptosis - involves flourscence microscopy (reply: 6)
2712. RFLP - reading gels (reply: 1)
2713. Regeneration of plasmid preparation columns - Has anyone a protocol? (reply: 1)
2714. ligation never works even for single cut vector!Why so!Help! - (reply: 7)
2715. removal of 3' overhangs - (reply: 4)
2716. DNase treatment in RNA isolation - protocol for DNase treatment (reply: 4)
2717. Southern Blot: which restriction enzyme? - (reply: 1)
2718. Site of transgene integration - (reply: 1)
2719. gDNA salvage for PCR from mounted tissue sections - Is it possible? (reply: 1)
2720. two plasmids of the same protein with tags on N and C-terminus. - how can I make use of them? (reply: 3)
2721. Reamplification of cDNA - (reply: 6)
2722. Pulse-Chase - band running below molecular weight (reply: 1)
2723. adaptors for cloning - how it works? (reply: 6)
2724. Site Directed Mutagenesis within CpG rich promoter - (reply: 4)
2725. Argggg! Contamination - its eating me alive!!! (reply: 9)
2726. RNA gel shift - (reply: 1)
2727. FISH section lost from sllide - (reply: 4)
2728. Random Primers vs Oligo(dT) primers? - (reply: 3)
2729. IPTG concentratoin - (reply: 1)
2730. smears on gel.. - REASONS (reply: 4)

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