3781. to purify these PCR products or not? - preparation for restriction digest (reply: 5)
3782. Strange analytical gel for miniprep - (reply: 4)
3783. How many mismatches in point mutation primers? - (reply: 3)
3784. RNA Isolation with RNeasy - (reply: 6)
3785. luciferase reporter vector - promega (reply: 1)
3786. staphylococcus DNA extraction - (reply: 2)
3787. HindIII digestion - (reply: 1)
3788. how to effectively recover 40 bp-long DNA fragment from restricted reaction - ask for suggestion (reply: 1)
3789. how many bases are needed upstream of restriction site? - (reply: 5)
3790. translation initiation - scannning of mRNA by ribosome machinery (reply: 1)
3791. Taqman for low levels of a gene (LacZ gene)in tissue - (reply: 1)
3792. Softwares and hardwares used for scanning gel electrophoresis banding pattern - (reply: 1)
3793. Direct DNA sequencing - sequencing without PCR product (reply: 6)
3794. Software available for Multiplex PCR primer designing - (reply: 3)
3795. Trizol RNA isolation - (reply: 1)
3796. Homologous recombination - (reply: 2)
3797. Whats the best media to get the fastest E.coli growth at a low cost? (only conce - (reply: 4)
3798. Picking stable clones by RT-PCR.. - (reply: 2)
3799. PCR of ligation products - (reply: 8)
3800. single primer PCR - use a single primer to amplify regions between inverted repeats (reply: 1)
3801. TA Cloning - (reply: 4)
3802. May I just use conventional E.Coli strain? - (reply: 2)
3803. Antisense Oligo nucleotide designing - (reply: 2)
3804. Transfering a DNA construct from one vector to another - in frame/not in frame? (reply: 2)
3805. DNA Purification from gels - (reply: 4)
3806. multiplex pcr trouble - some bands won't amplify (reply: 16)
3807. Hand-held UV wand: the most useful thing ever? - Gel electrophoresis, southerns and northerns (reply: 7)
3808. how do you inactivate the enzymes after digestion? - (reply: 17)
3809. Why is TE frequently used as buffer for DNA solutions? - (reply: 1)
3810. pQE30 - protein expression (reply: 1)

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