2191. digoxygenin-labelled primer - (reply: 3)
2192. transfection - dna chloroform (reply: 1)
2193. gene delivery lipofectamine - lipoplexes acetone (reply: 4)
2194. Agarose Gel Electrophoresis - Troubleshoot (reply: 12)
2195. DNA conc for PCR - (reply: 1)
2196. Cloning 5kb into PBlueScript (2.9kb)? - (reply: 5)
2197. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
2198. enygmatic restriction by BamH1 - (reply: 8)
2199. How to determine 3' end sequence of bacterial gene - (reply: 6)
2200. Origin of replication - more, better ? (reply: 6)
2201. about the lentivector production - (reply: 3)
2202. Old PCR publication - help! - (reply: 2)
2203. C group Plasmids? - (reply: 2)
2204. freezing of primers - (reply: 2)
2205. Primers with RE sites - (reply: 2)
2206. Phusion kit altering my restriction sites only - (reply: 5)
2207. Top 10 vs DH5α? - TOPO TA cloning question … (reply: 4)
2208. How long to store 1% agarose gels? - (reply: 11)
2209. Smeared PCR product - ISSR marker screening (reply: 1)
2210. Extraction of genomic DNA from 10 fold serial dilution of cells. - (reply: 5)
2211. Restriction with two incompatible enzymes - (reply: 4)
2212. SV40 early promoter read-through - (reply: 3)
2213. Not sure with cloning - (reply: 2)
2214. Annealing two Primers - PCR (reply: 7)
2215. GAA repeat expansion of Friedreich's ataxia (FA) - How to identify and size expansion? (reply: 3)
2216. DNA extraction from mouse ear punch - (reply: 2)
2217. DNA extraction from rodent tails - (reply: 8)
2218. what can i do with this pcr? - amplification of cDNA , with primers tm 50 and 67 (reply: 7)
2219. protoplast fusion - (reply: 3)
2220. low OD260/280 ratio for RNA extraction with TRIzol - (reply: 5)

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