4501. Different Tm - (reply: 2)
4502. restriction of pbluescript - restriction of pbluescript (reply: 4)
4503. Maximum insert size for pUC - (reply: 1)
4504. DNA/RNA ratio - techniques (reply: 1)
4505. Total RNA from lymphocytes - Problems with RNA yield (reply: 2)
4506. Competent cell - How good does it need to be? (reply: 4)
4507. Has my ligation work? - (reply: 7)
4508. How ethanol precipitation works? - (reply: 2)
4509. subcloning - (reply: 7)
4510. Purification of small DNA molecules - (reply: 6)
4511. Mysterious gain in vector size after cloning! - (reply: 1)
4512. Pilot-problems - (reply: 1)
4513. Blunt-Cohesive ends ligation - Blunt-Cohesive ends ligation (reply: 2)
4514. Primers design: how to check for mispriming? - (reply: 2)
4515. Internal Control RNA & RT-PCR - (reply: 1)
4516. RT-PCR - RT_PCR (reply: 2)
4517. PCR from genomic DNA - (reply: 6)
4518. Deletion Constructs w/Quik Change 2 Kit problemos - no colonies forming 14aa consecutive deletion (reply: 4)
4519. restriction enzyme inactivation - How do you inactivate restriction enzymes? (reply: 4)
4520. EMSA gel - dirty bands! (reply: 7)
4521. concept of the molar ratio - concept of the molar ratio (reply: 4)
4522. site-directed mutagenesis - (reply: 10)
4523. I-SceI meganuclease - restriction trouble of BAC DNA (reply: 1)
4524. Ligation problems - (reply: 2)
4525. question about DNA precipitation - (reply: 2)
4526. Co-transfection - (reply: 4)
4527. standard curves - real time pcr (reply: 3)
4528. Ligation of two oligos into a vector - (reply: 4)
4529. why mRNA difficult to isolate - (reply: 5)
4530. Miniprep ! Can it bug you? - (reply: 1)

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