2101. RNA extraction from parasite - (reply: 2)
2102. quantitative and semi quantitative assay - bit confusion (reply: 1)
2103. Cloning & Expression - Cloning & Expression (reply: 7)
2104. ChIP assay! - (reply: 1)
2105. Strain? isolate? - (reply: 1)
2106. Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
2107. Dephosphorylation - (reply: 4)
2108. RNA isolation from mouse heart tissue - (reply: 2)
2109. Titration? - (reply: 2)
2110. Ever heard not of Prime Dimers but Primer Multimers? - (reply: 1)
2111. YEAST EXPRESSION - (reply: 1)
2112. How to interpret a Western Blot? - (reply: 5)
2113. Protein isolation - Small size His-tag Protein isolation (reply: 4)
2114. wht is the best way to extract RNA from oocytes stages - (reply: 1)
2115. A. R. grade isopropanol - (reply: 4)
2116. PCR in 96-Well Plate - (reply: 4)
2117. Mix for sequencing - Big Dye buffer necessary or not? (reply: 3)
2118. DNA isolation method form CSF - (reply: 2)
2119. Immunoscreening difficulty - (reply: 1)
2120. make PCR template blunt ended - (reply: 2)
2121. autoclaving tubes for PCR/RT-PCR - (reply: 5)
2122. contaminated gel eluted DNA fragment - (reply: 8)
2123. What is wrong with my EcoRI digestion? - Can it attribute to the star activity? (reply: 6)
2124. plasmid molar concetration/copy number - (reply: 2)
2125. AT rich PCR problems - anyone experienced with tetramethylammonium chlroide? (reply: 3)
2126. Quickchange site directed mutagenesis/PCR problem - Transformation isn't working :( (reply: 8)
2127. how would you detect a point mutation in the genome? PCR? - (reply: 3)
2128. faint band of interest & primer-dimer prob - (reply: 5)
2129. about size of genomic DNA - (reply: 5)
2130. PCR contamination in NoRT Control - I need advice (reply: 1)

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