help on transformation - BAC clones transformation (Jun/11/2005 )

Hi,

This is my first time working with BAC clones. I have tried using heat-shock method to transform BAC into DH5a competent cells. But it doesn't work!

I read that electroporation is the usual preferred method for transformation of BAC clone, but is there other methods around that i can use?

Thanks,

-arnotts10-

My friend worked on YAC and BAC and she said transformation rate into E.coli cells was quite low.

I asked her just to prolong the on-ice incubation time upto 1hour, she did and got a better result, you may try it!

Goodluck and let us know if you get it done!

-dtle-

QUOTE (dtle @ Jun 12 2005, 10:49 PM)

thanks for your reply.
Do u mean, the on ice incubation before heat shock or after heat shock at 42 degrees?

-arnotts10-

QUOTE (arnotts10 @ Jun 12 2005, 06:12 PM)

thanks for your reply.
Do u mean, the on ice incubation before heat shock or after heat shock at 42 degrees?

Sorry for insufficient info, after heat shock at 42oC!

-dtle-

QUOTE (dtle @ Jun 13 2005, 02:24 PM)

QUOTE (arnotts10 @ Jun 12 2005, 06:12 PM)

thanks for your reply.
Do u mean, the on ice incubation before heat shock or after heat shock at 42 degrees?

Sorry for insufficient info, after heat shock at 42oC!

thanks.. i will give it a try and let u know if it works for mine..

-arnotts10-