the range of protein amount for loading - western blotting (Sep/11/2005 )

What is the range of protein amount you load on your well for western blot?

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I generally do 25-50ug of cell lysate.

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usually about 20ug - 50ug of the sample is added

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It depends on the size of the gel:
20-50 microgram for a small gel and up to 100 microgram for a large gel (16-18 cm long)

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10ug/well for brain tissue lysate

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