Path length measuring protein concentration - (Nov/10/2005 )

Hi all,

I wonder what should I do to calculate a protein concentration from its absorbance if the path length is 0.1 cm.

Thanks in advance

-celvas-

Multiply by 10, unless the software does already does it for you.

QUOTE (celvas @ Nov 10 2005, 12:49 PM)

Hi all,

I wonder what should I do to calculate a protein concentration from its absorbance if the path length is 0.1 cm.

Thanks in advance

-Cole-

I agree, based on Beer's law, from a physical view.

-shelf-

QUOTE (celvas @ Nov 10 2005, 05:49 PM)

Hi all,

I wonder what should I do to calculate a protein concentration from its absorbance if the path length is 0.1 cm.

Thanks in advance

You have to se A= ExCxl
A: Absornace
E: extinction coefficient
C: concentration
l: path length

-elisabeth Kamp-