why get rid of imidazole in his tag protein purification - (Dec/07/2005 )
could be stupid question. Even I did dialysis to get rid of it, but I am not sure why should I get rid of it. If it stays, can I send this sample for raising antibody?
And if the protein contains imidazole, is it affect the protease activity?
Thanks a lot 
Interesting question! Don't know if imidazole is toxic to animals. Maybe? Imidazole is a ring molecule similar to amino acid histidine. If there is a catalytic triad in your active site that requires histidine, I do know by experience that imidazole can affect your assays.
Cindy
And if the protein contains imidazole, is it affect the protease activity?
Thanks a lot
so what are the reasons to get rid of imidazole? with the imidazole in it , could it affect the protein use as an immugen to get antibody?
Thanks fella
To rise immune reaction people used to utilize the pure protein.
Nobody knows, how imidazole could modify immune reaction. There are some agents which burst the immune responce (adiuvants). But imidazole could as well act like immunosupressor - therefore give no antibody responce at all. When it is not studied, better to dialyse it out and be sure, that you will have nice antibodies against your protein ( and not mostly against imidazole for example).