protein loss in sterile filtration - suggest the 0.22 micron filter (Jan/12/2006 )
dear all
i have been loosing my protein up to 30% during sterile filtration,where as with less hydrophobic and hydro phylic natured proteins. i need to fgilter upto 2 litres of final protein bulk.
please any body can suggest good vendor for 0.22 filters with less adsorbant .
thank u
-kasa-
QUOTE (kasa @ Jan 12 2006, 10:02 AM)
dear all
i have been loosing my protein up to 30% during sterile filtration,where as with less hydrophobic and hydro phylic natured proteins. i need to fgilter upto 2 litres of final protein bulk.
please any body can suggest good vendor for 0.22 filters with less adsorbant .
thank u
i have been loosing my protein up to 30% during sterile filtration,where as with less hydrophobic and hydro phylic natured proteins. i need to fgilter upto 2 litres of final protein bulk.
please any body can suggest good vendor for 0.22 filters with less adsorbant .
thank u
-Asthma-
Hi,
Millipore is usually really good for filters. You will need to make sure you buy a non- or low-binding filter. Likely that your protein may be sticking to the filter.
Best
-Asthma-
QUOTE (kasa @ Jan 12 2006, 10:32 PM)
dear all
i have been loosing my protein up to 30% during sterile filtration,where as with less hydrophobic and hydro phylic natured proteins. i need to fgilter upto 2 litres of final protein bulk.
please any body can suggest good vendor for 0.22 filters with less adsorbant .
thank u
i have been loosing my protein up to 30% during sterile filtration,where as with less hydrophobic and hydro phylic natured proteins. i need to fgilter upto 2 litres of final protein bulk.
please any body can suggest good vendor for 0.22 filters with less adsorbant .
thank u
Nitrocellulose membrane filters would adsorb less protein i suppose. U using that one?
-cheeztoast-
We have those filters in the lab, I will have a look to tell you.
I don't remember the name.
-macedo-