proteolytic digests - which enzymes cut where.... (Jan/31/2006 )
hello all, im trying to understand the basis of proteolysis. For example trypsin cuts this amino acid, chymotrypsin cuts that amino acid.....just cant find any good reference of some proteolytic enzymes. Am I too simplyfyin it? ...thanx a lot for any help....
just for starting the discussion :
Trypsin cuts carboxy side of Lys and arginine. Chymotrypsin cuts at Tyr and Phe link)
if you have other specific proteases you can search google by typing "name-of-protease cuts" and you'll find a relevant web page.
i haven't found a summing up table for that yet.
hope that helps.
Attached you find a pic from my presentation for mass spec. Ther are some enzymes with there specific digestion...
Oli
thanx everyone....after some searching i have also found this site helpful....
http://merops.sanger.ac.uk/cgi-bin/merops.cgi?id=m9
ok this site is also very good....
The University of Reading
Department of Food Science and Technology
BSc Module FS460
Investigating Protein Structure and Function
please copy it to google...cant find URL...
The University of Reading
Department of Food Science and Technology
BSc Module FS460
Investigating Protein Structure and Function
please copy it to google...cant find URL...
This is the link
Oli
thanx Oli...