so my protein has degraded....why am I seeing higher bands? - (May/18/2006 )

i was just explaning to my prof that my protein has degraded as I left it at 4C....and was showing him the PAGE....but there are bigger bands also not just smaller ones... ....what could those possibly be?????

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depends on size - could it be multimers of protein or do you cook sample for about 5 min 100°C (which should separate them)?

Rekombinant Protein in E.coli if I got it right - so can't be posttranslational...

Your protein isn't his-tagged so you could do an anti-his Western to be sure?

Sorry but haven't been following through your other posts...

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it is, most likely, denatured, insoluble (or nearly) aggregates of your protein.

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thank you guys for your explanations.... ....ill use them in the next meeting....

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