how to calculate pmole ends? - (May/12/2006 )
hello everybody, I wanna know how to calculate pmole ends for my Plasmid of 5.4kb with one site for EcoR1 in it, as i wanna do dephosphorylation with CIAP.
i use it like
pmole ends=pmol DNA (No of cuts)(2), but dont know how to calculate pmoles of my Vector that is 5.4kb and concentration is 1ug.
I will appreciate
Thanks
-bushra-
biomath calculator or simiar do the job.
-fred_33-