what buffer will prevent the precipitation of protein at -20? - (Aug/06/2006 )
can somebody recommend me some buffer that prevent or help the precipitation of protein in freezer?
-cnbeatles-
What are you using now?
-swanny-
you could add some glycerol?
but it depends on your protein, on the buffer that's already in there, and what you plan to do with the protein later...
-aimikins-
well i would suggest you some buffer related to those for restriction enzymes...
You can add 50%glycerolin your protein buffer. But may be too high, and i don't have tests for minimum glycerol amount for not freezing at -20°.
-fred_33-
One time I add 20% glycerol to purified T7 polymerase, it precipitated shortly. I lost everything.
-genehunter-1-
you may also have a look a commercially available related proteins for checking in which buffer they're compatible...
-fred_33-